1. What is the RCF Equation?

The RCF (Relative Centrifugal Force) equation converts RPM (revolutions per minute) to g-force. It provides a standardized way to compare centrifugation forces across different centrifuges with varying rotor sizes.

2. How Does the Calculator Work?

The calculator uses the RCF equation:

Where:

• \( RPM \) — Revolutions per minute
• \( Radius \) — Rotor radius in meters
• \( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The equation accounts for the squared relationship between RPM and centrifugal force, with the radius determining the effective force at a given speed.

3. Importance of RCF Calculation

Details: Accurate RCF calculation is crucial for standardizing centrifugation protocols across different equipment, ensuring reproducible results in laboratory procedures.

4. Using the Calculator

Tips: Enter RPM in revolutions per minute and radius in meters. All values must be valid (RPM > 0, radius > 0).

5. Frequently Asked Questions (FAQ)

Q1: Why convert RPM to RCF?
A: RCF provides a standardized measure of centrifugal force that accounts for differences in rotor size, allowing for consistent protocols across different centrifuges.

Q2: What are typical RCF values used in centrifugation?
A: RCF values range from a few hundred g for gentle separations to over 100,000 g for high-speed ultracentrifugation.

Q3: How do I measure the radius accurately?
A: Measure from the center of the rotor to the bottom of the tube holder when the rotor is at rest. Consult the centrifuge manual for specifications.

Q4: Are there limitations to this equation?
A: The equation assumes ideal conditions and may not account for factors like tube angle, fluid dynamics, or non-uniform acceleration.

Q5: Can I use this for any type of centrifuge?
A: Yes, the equation is universal for all centrifuges, but always verify with manufacturer specifications for critical applications.